| | | |
| March 9, 2025 | W | St. Louis City SC 2 3 at North Texas SC 0 | Final |
| March 14, 2025 | W | St. Louis City SC 2 1 at Sporting Kansas City II 0 | Final |
| March 23, 2025 | W | Sporting Kansas City II 0 at St. Louis City SC 2 2 | Final |
| March 30, 2025 | T | Austin FC II 0 at St. Louis City SC 2 0 | Final (PK 4-5) |
| April 6, 2025 | L | Colorado Rapids 2 1 at St. Louis City SC 2 0 | Final |
| April 13, 2025 | L | St. Louis City SC 2 1 at Ventura County FC 3 | Final |
| April 26, 2025 | L | St. Louis City SC 2 1 at Tacoma Defiance 4 | Final |
| May 4, 2025 | | Houston Dynamo 2 at St. Louis City SC 2 | 5:00 p.m. |
| May 11, 2025 | | St. Louis City SC 2 at The Town FC | 8:00 p.m. |
| May 18, 2025 | | St. Louis City SC 2 at Minnesota United FC 2 | 4:00 p.m. |
| May 23, 2025 | | Portland Timbers 2 at St. Louis City SC 2 | 8:00 p.m. |
| June 4, 2025 | | North Texas SC at St. Louis City SC 2 | 8:00 p.m. |
| June 15, 2025 | | St. Louis City SC 2 at Colorado Rapids 2 | 8:00 p.m. |
| June 22, 2025 | | The Town FC at St. Louis City SC 2 | 7:00 p.m. |
| June 29, 2025 | | Houston Dynamo 2 at St. Louis City SC 2 | 7:00 p.m. |
| July 6, 2025 | | St. Louis City SC 2 at Real Monarchs | 9:30 p.m. |
| July 11, 2025 | | St. Louis City SC 2 at Austin FC II | 8:30 p.m. |
| July 16, 2025 | | Ventura County FC at St. Louis City SC 2 | 8:00 p.m. |
| July 27, 2025 | | Minnesota United FC 2 at St. Louis City SC 2 | 7:00 p.m. |
| August 6, 2025 | | St. Louis City SC 2 at Vancouver Whitecaps FC 2 | 9:00 p.m. |
| August 10, 2025 | | Colorado Rapids 2 at St. Louis City SC 2 | 7:00 p.m. |
| August 16, 2025 | | St. Louis City SC 2 at North Texas SC | 8:45 p.m. |
| August 24, 2025 | | Sporting Kansas City II at St. Louis City SC 2 | 7:00 p.m. |
| August 31, 2025 | | St. Louis City SC 2 at Houston Dynamo 2 | 8:00 p.m. |
| September 13, 2025 | | Minnesota United FC 2 at St. Louis City SC 2 | 7:00 p.m. |
| September 21, 2025 | | St. Louis City SC 2 at Austin FC II | 8:30 p.m. |
| September 28, 2025 | | LAFC2 at St. Louis City SC 2 | 5:00 p.m. |
| October 5, 2025 | | St. Louis City SC 2 at Portland Timbers 2 | 4:00 p.m. |
St. Louis City SC 2 Schedule
