| | | |
| March 8, 2025 | W | Louisville City FC 2 at Charleston Battery 1 | Final |
| March 16, 2025 | T | Louisville City FC 1 at Birmingham Legion FC 1 | Final |
| March 22, 2025 | W | Loudoun United FC 0 at Louisville City FC 2 | Final |
| March 29, 2025 | W | Detroit City FC 0 at Louisville City FC 2 | Final |
| April 5, 2025 | T | Louisville City FC 1 at Sacramento Republic FC 1 | Final |
| April 19, 2025 | W | Louisville City FC 2 at Tampa Bay Rowdies 1 | Final |
| April 26, 2025 | W | Richmond Kickers 1 at Louisville City FC 4 (Jagermeister Cup) | Final |
| May 1, 2025 | | Lexington Sporting Club at Louisville City FC | 8:00 p.m. |
| May 10, 2025 | | Pittsburgh Riverhounds SC at Louisville City FC | 7:30 p.m. |
| May 17, 2025 | | Louisville City FC at Hartford Athletic | 7:00 p.m. |
| May 24, 2025 | | Louisville City FC at Monterey Bay FC | 10:00 p.m. |
| May 31, 2025 | | Louisville City FC at Loudoun United FC | 6:00 p.m. |
| June 7, 2025 | | Tampa Bay Rowdies at Louisville City FC | 8:00 p.m. |
| June 11, 2025 | | Rhode Island FC at Louisville City FC | 8:00 p.m. |
| June 14, 2025 | | Birmingham Legion FC at Louisville City FC | 8:00 p.m. |
| June 20, 2025 | | Louisville City FC at North Carolina FC | 7:30 p.m. |
| June 25, 2025 | | Louisville City FC at Loudoun United FC | 6:00 p.m. |
| June 28, 2025 | | North Carolina FC at Louisville City FC | 8:00 p.m. |
| July 4, 2025 | | Louisville City FC at Colorado Springs Switchbacks FC | 9:00 p.m. |
| July 19, 2025 | | FC Tulsa at Louisville City FC | 8:00 p.m. |
| July 26, 2025 | | Louisville City FC at Lexington Sporting Club | 7:00 p.m. |
| August 2, 2025 | | North Carolina FC at Louisville City FC | 8:00 p.m. |
| August 9, 2025 | | Charleston Battery at Louisville City FC | 8:00 p.m. |
| August 16, 2025 | | Louisville City FC at Miami FC | 7:00 p.m. |
| August 23, 2025 | | New Mexico United at Louisville City FC | 8:00 p.m. |
| August 30, 2025 | | Louisville City FC at Indy Eleven | 7:00 p.m. |
| September 6, 2025 | | Louisville City FC at Rhode Island FC | 4:00 p.m. |
| September 13, 2025 | | Louisville City FC at Pittsburgh Riverhounds SC | 7:00 p.m. |
| September 20, 2025 | | Las Vegas Lights FC at Louisville City FC | 7:30 p.m. |
| September 27, 2025 | | Louisville City FC at Detroit City FC | 7:00 p.m. |
| October 4, 2025 | | Indy Eleven at Louisville City FC | 7:30 p.m. |
| October 11, 2025 | | Miami FC at Louisville City FC | 7:30 p.m. |
| October 18, 2025 | | Louisville City FC at Orange County SC | 10:00 p.m. |
| October 25, 2025 | | Hartford Athletic at Louisville City FC | 7:30 p.m. |
Louisville City FC Schedule
