| | | |
| February 22, 2025 | W | New York Red Bulls 0 at FC Cincinnati 1 | Final |
| March 1, 2025 | L | FC Cincinnati 1 at Philadelphia Union 4 | Final |
| March 8, 2025 | W | Toronto FC 0 at FC Cincinnati 2 | Final |
| March 15, 2025 | L | FC Cincinnati 0 at Charlotte FC 2 | Final |
| March 22, 2025 | T | Atlanta United FC 2 at FC Cincinnati 2 | Final |
| March 29, 2025 | W | FC Cincinnati 2 at Nashville SC 1 | Final |
| April 5, 2025 | W | New England Revolution 0 at FC Cincinnati 1 | Final |
| April 12, 2025 | W | FC Cincinnati 1 at D.C. United 0 | Final |
| April 19, 2025 | W | FC Cincinnati 3 at Chicago Fire FC 2 | Final |
| April 26, 2025 | | Sporting Kansas City at FC Cincinnati | 2:30 p.m. |
| May 4, 2025 | | FC Cincinnati at New York City FC | 3:00 p.m. |
| May 10, 2025 | | Austin FC at FC Cincinnati | 7:30 p.m. |
| May 14, 2025 | | FC Cincinnati at Toronto FC | 7:30 p.m. |
| May 17, 2025 | | FC Cincinnati at Columbus Crew SC | 7:00 p.m. |
| May 25, 2025 | | FC Cincinnati at Atlanta United FC | 7:00 p.m. |
| May 28, 2025 | | FC Dallas at FC Cincinnati | 7:30 p.m. |
| May 31, 2025 | | D.C. United at FC Cincinnati | 7:30 p.m. |
| June 14, 2025 | | FC Cincinnati at New England Revolution | 7:30 p.m. |
| June 25, 2025 | | FC Cincinnati at Club de Foot Montreal | 7:30 p.m. |
| June 28, 2025 | | FC Cincinnati at Orlando City SC | 7:30 p.m. |
| July 5, 2025 | | Chicago Fire FC at FC Cincinnati | 7:30 p.m. |
| July 12, 2025 | | Columbus Crew SC at FC Cincinnati | 7:30 p.m. |
| July 16, 2025 | | Inter Miami CF at FC Cincinnati | 7:30 p.m. |
| July 19, 2025 | | FC Cincinnati at Real Salt Lake | 9:30 p.m. |
| July 26, 2025 | | FC Cincinnati at Inter Miami CF | 7:00 p.m. |
| August 10, 2025 | | Charlotte FC at FC Cincinnati | 6:00 p.m. |
| August 16, 2025 | | FC Cincinnati at Portland Timbers | 10:30 p.m. |
| August 23, 2025 | | New York City FC at FC Cincinnati | 7:30 p.m. |
| August 30, 2025 | | Philadelphia Union at FC Cincinnati | 7:30 p.m. |
| September 13, 2025 | | Nashville SC at FC Cincinnati | 7:30 p.m. |
| September 20, 2025 | | FC Cincinnati at LA Galaxy | 10:30 p.m. |
| September 27, 2025 | | Orlando City SC at FC Cincinnati | 2:30 p.m. |
| October 4, 2025 | | FC Cincinnati at New York Red Bulls | 7:30 p.m. |
| October 18, 2025 | | Club de Foot Montreal at FC Cincinnati | 6:00 p.m. |
FC Cincinnati Schedule
