| | | |
| March 21, 2025 | W | Carolina Cobras 0 at Columbus Lions 25 | Final |
| March 28, 2025 | L | Columbus Lions 20 at Carolina Cobras 40 | Final |
| April 18, 2025 | L | Beaumont Renegades 44 at Columbus Lions 37 | Final |
| April 26, 2025 | L | Columbus Lions 38 at Colorado Spartans 42 | Final |
| May 3, 2025 | | Columbus Lions at Carolina Cobras | Cancelled |
| May 10, 2025 | | Shreveport Rouxgaroux at Columbus Lions | 7:00 p.m. |
| May 17, 2025 | | Columbus Lions at Beaumont Renegades | 7:00 p.m. |
| May 24, 2025 | | Columbus Lions at Wheeling Miners | 7:00 p.m. |
| May 31, 2025 | | Wheeling Miners at Columbus Lions | 7:00 p.m. |
Columbus Lions Schedule
